2× Hieff Canace™ AdvanceFast High-Fidelity PCR Master Mix (With Dye)
Ready-to-use high-fidelity PCR master mix with pre-added electrophoresis indicators for convenient DNA amplification and direct gel electrophoresis.
Product Information
| Catalogue Number | 1015-10164ES08 |
| Product Size | 5 × 1 mL |
| Product Format | 2× PCR Master Mix with Dye |
| Research Area | Molecular Biology |
| Dry Ice | Yes |
| Hazardous | No |
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product Description
2× Hieff Canace™ AdvanceFast High-Fidelity PCR Master Mix (With Dye) is a ready-to-use 2× premixed solution containing Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase, dNTPs, and an optimized buffer system with pre-added electrophoresis indicators.
The integrated electrophoresis indicators allow PCR products to be loaded directly onto an electrophoresis gel without requiring a separate loading dye step. The amplification products have blunt ends.
This master mix is designed to provide a convenient PCR workflow, combining high sensitivity, high specificity, and good stability. The reaction setup is simplified: primers and template DNA are added to the premix to prepare the amplification reaction.
A specific protective agent is also included to help maintain premix activity after repeated freezing and thawing.
Key Features & Benefits
- High-fidelity amplification: Contains AdvanceFast High-Fidelity DNA Polymerase for PCR applications requiring accurate DNA amplification.
- Ready-to-use 2× formulation: Includes polymerase, dNTPs, and optimized reaction buffer.
- Pre-added electrophoresis indicators: Enables direct loading of PCR products onto an electrophoresis gel.
- Blunt-ended PCR products: Amplification products are generated with flat ends.
- Simplified reaction setup: Add primers and template DNA to the master mix.
- Freeze-thaw stability: Formulated with a protective agent to help preserve activity after repeated freeze-thaw cycles.
Research Applications
- High-fidelity polymerase chain reaction (PCR)
- DNA fragment amplification
- Molecular biology research
- PCR product analysis by gel electrophoresis
- Research workflows requiring blunt-ended amplification products
Technical Specifications
| Polymerase | Hieff Canace™ AdvanceFast High-Fidelity DNA Polymerase |
| Concentration | 2× premixed formulation |
| Included Components | DNA polymerase, dNTPs, optimized buffer system, electrophoresis indicators, protective agent |
| PCR Product Ends | Blunt ends |
| Electrophoresis | Direct loading of PCR products |
| Pack Size | 5 × 1 mL |
Shipping & Storage Information
Shipping Condition: Dry Ice
Hazardous Status: No
Follow the manufacturer's instructions for storage, handling, and freeze-thaw management.